Relevance of Plasmodium falciparum Biomarkers in the Treatment and Control of Malaria

We report here a dual-ELISA method to measure Malaria biomarkers concurrently in the same aliquot of blood sample. A correlation between parasite numbers and ELISA values determined figures were used establish standard graph. Thick smears prepared from spiked samples also Giemsa stained density by microscopy (It was thereby possible undertake an objective comparison lactate dehydrogenase histidine- rich- proteins levels assessed aliquot). The presence is localized visualized fluorescent antibody techniques. Twenty-two patient retrospectively analyzed for pLDH HRPs their level quantified each Fresh malaria patients seeking care at healthcare facility endemic area then collected separated into plasma infected red cells parasitemia determined. which released circulating sequestered parasites provide more accurate picture indicate degree disease severity. Since known have short half-life compared histidine-rich proteins, its believed reflect on number metabolically active accurately reliable indicator clinical outcome. In contrast, histidine -rich accumulate measurable persist long after been treated cleared system. Best practice may now call pre-eminent role PCR plasmodium species confirmation, continued newer protein assays epidemiological studies control, preferential use management disease. Data presented as well naturally evidence that splitting whole RBC-pellet plasma, determination split sample fractions gives true present humans this improve accuracy models attempt predict burdens accurately, so correlations.